Ancestry Research in Portugal
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 | Manuel I of Portugal, King of Portugal  (1469-1521)
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 | Maria of Aragon, Queen of Portugal (1482-1517)
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| Isabella of Portugal, Holy Roman Empress (1503-1539)  |
| Beatrice of Portugal, Duchess of Savoy (1504-1538) |
 | Luís of Portugal, Duke of Beja (1506-1555) |
| Ferdinand of Portugal, Duke of Guarda (1507-1534) |
| Maria of Portugal (1511-1513) |
| Henry I of Portugal, King of Portugal (1512-1580) |
 | Duarte I Portugal, 4th Duke of Guimarães (1515-1540) |
| António of Portugal (1516-1516) |
| Catherine of Austria (1507-1578) |
| John III of Portugal, King of Portugal (1502-1557)
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| Afonso of Portugal, Prince of Portugal (1526-1526) |
| Maria Manuela of Portugal, Princess of Asturias (1527-1545) |
| Manuel of Portugal, Prince of Portugal (1531-1537) |
| Philip of Portugal, Prince of Portugal (1533-1539) |
| João Manuel of Portugal, Prince of Portugal (1537-1554) |
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