Ancestry Research in Portugal
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 | Charles V of Austria, Holy Roman Emperor  (1500-1558)
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 | Isabella of Portugal, Holy Roman Empress (1503-1539)
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 | John III of Portugal, King of Portugal (1502-1557)
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| Catherine of Austria (1507-1578)
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| Afonso of Portugal, Prince of Portugal (1526-1526) |
| Manuel of Portugal, Prince of Portugal (1531-1537) |
| Philip of Portugal, Prince of Portugal (1533-1539) |
| João Manuel of Portugal, Prince of Portugal (1537-1554) |
 | Philip II of Spain, King of Spain (1527-1598)
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| Maria Manuela of Portugal, Princess of Asturias (1527-1545)
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| Carlos of Spain, Prince of Asturias (1545-1568) |
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