Ancestry Research in Portugal
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 | John III of Portugal, King of Portugal  (1502-1557)
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 | Catherine of Austria (1507-1578) |
| Afonso of Portugal, Prince of Portugal (1526-1526) |
| Manuel of Portugal, Prince of Portugal (1531-1537) |
| Philip of Portugal, Prince of Portugal (1533-1539) |
| João Manuel of Portugal, Prince of Portugal (1537-1554) |
| Philip II of Spain (1527-159) |
| Maria Manuela of Portugal, Princess of Asturias (1527-1545)
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